Not known Factual Statements About flow cytometers
Not known Factual Statements About flow cytometers
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At the moment, the early signals were being dismissed as particles, nevertheless the retrospective evaluation demonstrated shared attributes While using the problematic cells in culture. Tracing the infection helped the lab build additional laboratory processes for screening and safety of assay-important mobile strains.
As samples are acquired to the Attune CytPix Flow Cytometer, the higher-velocity brightfield camera captures and stores photographs of detected gatherings at a charge up to six,000 photos for each 2nd, based on the flow price and image dimensions. For better versatility, Attune Cytometric Computer software lets you alter the picture capture frequency as desired and select a certain gate for graphic capture, if required.
Incorporate acoustic concentrating know-how with superior-parameter Examination for adaptable spectral unmixing and standard workflows.
Find new insights into your experimental facts by visualizing populations applying combined fluorescence and morphology info. Our computer software interface wizards enable downsampling, concatenation (merged files) and dimensionality equipment which are constructed in to the computer software for an intuitive, streamlined details analysis knowledge.
"An image isn't quite truly worth 1000 dots, nonetheless it truly helps you to see illustrations or photos. It is great to determine images that correlate with staining. As an example, AnnexinV-good cells which are morphologically distinctive than healthful cells, or CD14+ monocytes that happen to be larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Additionally, it can be illuminating to see the number of doublets slip by means of standard singlet gates, and great to verify the position of sudden double-good gatherings in my Examination."
Pair your Attune flow cytometer with the autosampler for elevated workflow efficiency, and insert robotic integration for optimum throughput.
Regular picture high quality even at superior throughput Acoustic concentrating and also a significant-speed digicam Mix to picture these T cells consistently at minimal or superior flow prices. Simply change concentration and camera configurations to fulfill experimental needs.
The Attune NxT cytometer will be the responsible workhorse instrument your lab can depend upon for regular and reliable general performance. The Attune CytPix product contains a significant-velocity brightfield digital camera and AI-pushed impression Examination software, with modern updates which include equipment learning equipment and an intuitive consumer interface, enabling Improved info precision, accuracy, and a lot quicker insights into sample biology.
End users can see celebration visuals in genuine-time to substantiate sample good quality, enhance protocols, and record the morphology of every mobile populace discovered for Examination. Imaging capture also Added benefits from acoustic focusing, which helps to posture the cells making sure that a sharp, centered impression is obtained.
AI-pushed automated impression analysis Along with the Attune Cytometric Application translates event characteristics into distinct morphology parameters that can be combined with conventional fluorescence and scatter parameters to reveal highly reproducible populace alterations and special subsets.
We Formerly shown the strength of imaging Car or truck-T/Ramos cell interactions. Let’s check out just the population of biggest desire, the double positive functions, to learn more. We can now use prolonged morphological parameters (below, circularity and skewness of intensity) to even further analyze the characteristics of such populations and refine gating on these events, raising knowledge robustness.
Leverage the power of State-of-the-art technology and innovation to accelerate your discoveries. Believe in in the Attune Flow Cytometer family members to deliver the trustworthiness, velocity, and accuracy that established your perform apart.
Versatile payment system from Agilent Monetary Remedies help you receive the essential instrumentation you may need for speedy task, or to mature get more info your lab.
Gating on these prolonged parameters allows you to speedily and accurately discover populations of interest and confirm gating strategy with little if any guide assessment. The Attune computer software presents use of point out-of-the-art machine Understanding Evaluation instruments in an expertly created person interface, rendering it exceptionally simple to operate.
Because the cell line had previously been analyzed to the Attune CytPix Flow Cytometer, the researchers went again to the images and were capable to document the microbial infection at least five days previously.
Agilent Worth Assure guarantees you at the very least a decade of instrument use from the date you buy. Or else, We'll credit rating you With all the residual price of the program towards an upgraded design.
What appeared to be a homogeneous population is definitely far more assorted—an Perception that ought to be regarded when interpreting outcomes.